ALLODX / RESEARCH
iPSC-Derived Podocyte Functional Studies
Use iPSC-derived podocyte models to investigate how serum components and other factors affect cell morphology, injury, and function.
Why use iPSC-derived podocytes?
Podocytes are integral to the glomerular filtration barrier, and their morphological and functional changes are relevant to proteinuric kidney diseases. Published studies have differentiated human iPSCs into marker-expressing podocyte models with cellular processes for injury and glomerular-function research.
Published studies provide model background and do not establish that this service reproduces every reported maturation feature, chip function, or performance metric.
From pilot experiments to functional studies
- Define iPSC source and study groups
- Differentiate cells and assess their state
- Replate and culture cells for serum-component or other treatments
- Assess morphology, injury, and functional endpoints
The current AlloDx plan begins with an exploratory pilot linking iPSC culture, podocyte induction, replating, and post-treatment observation. Cell source, induction batches, and full-study scale are agreed through project discussions and pilot results.
Potential endpoints
Markers and morphology
Staining for markers such as nephrin and KIRREL1 (NEPH1), alongside intercellular junctions and cellular processes.
Explore service →Injury and function
Design studies of serum-component-induced injury or apoptosis and consider functional readouts such as albumin uptake.
Explore service →Molecular analyses
Depending on pilot findings, integrate qPCR or transcriptomic analyses with morphology and function.
Explore service →Study scope and planning
These endpoints are not all included by default. Marker combinations, imaging, and molecular assays are agreed individually; fixed cell yields, delivery schedules, or clinical risk-prediction performance are not guaranteed.
Technical background and references
Services on this page are for research use. Project scope, sample requirements, and deliverables are defined in the agreed study plan.
Start with your research question
Tell us your research area, sample type, study groups, and intended outcomes. Together, we will define the technical plan and deliverables.
Discuss your research needs ↗